Protocols

Sampling and transporation

We follow standardized procedures for field sample collection to ensure minimal contamination and consistency across samples. The linked protocol describes required materials, collection techniques, and storage guidelines.
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DNA extraction

This protocol outlines the steps for extracting high-quality DNA suitable for downstream amplicon and shotgun sequencing. It includes buffer preparation, lysis conditions, and cleanup methods.
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Library preparation

16S amplicon

This document details PCR primer design, amplification conditions, and barcoding strategy specific to 16S rRNA gene sequencing. It is optimized for both Illumina and ONT platforms.
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18S amplicon

Our 18S workflow targets eukaryotic microbial communities with a universal primer pair and optimized thermal cycling. It also includes adapter ligation and cleanup.
📄 View 18S Amplicon Protocol ⬇️ Download PDF

ITS amplicon

This protocol targets fungal ITS regions using well-established primers. It includes PCR amplification and quality control steps tailored for environmental samples.
📄 View ITS Amplicon Protocol ⬇️ Download PDF

Shotgun metagenomics protocol

We use a PCR-free library preparation kit for shotgun metagenomic sequencing. The protocol describes DNA shearing, end repair, and adapter ligation for high-throughput platforms.
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Data analysis

This protocol covers raw sequence QC, host read removal, taxonomic profiling, and functional annotation. Tools and databases are listed with usage tips and links.
📄 View Data Analysis Protocol